YB90120Hu01

Stem Cell Factor (SCF)

Organism: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

1th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Protein Names: Stem Cell Factor

Gene Names: SCF

Size: 100µg

Source: Recombinant

Expression Host: E.coli

Function: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

Subcellular Location: Cell membrane; Single-pass type I membrane protein; Secreted

[ PROPERTIES ]

Residues: Glu26-Ala189 (Accession # P21583), with a N-terminal Met.

Grade & Purity: >97%, 18.6 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 18.6 kDa

[ PREPARATION ]

Reconstitute in PBS.

[ STORAGE AND STABILITY ]

Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term storage. Avoid repeated freeze/thaw cycles.

Valid period: 12 months stored at -80oC.

[ REFERENCES ]

1.Erlandsson, A., et al. (2004) Exp. Cell Res. 301:201.

2.Martin, F.H., et al. (1990) Cell 63:203.

3.Lemmon, M.A., et al. (1997) J. Biol. Chem. 272:6311.

4.Arakawa, T., et al. (1991) J. Biol. Chem. 266:18942.

5.Kapur, R., et al. (2002) Blood 100:1287.

5.Kanellakis, P., et al. (2006) Cardiovasc. Res. 70:117.

6.Yoshida, H., et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.

8.Ashman, L.K., (1999) Int. J. Biochem. Cell Biol. 31:1037.

9.Sette, C., et al. (2000) Int. J. Dev. Biol. 44:599.

10.Wang, C.H., et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.

11.Bashamboo, A., et al. (2006) J. Cell Sci. 119:3039.

12.Reber, L., et al. (2006) Eur. J. Pharmacol. 533:327.

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