YBC105Mu01 10µg

Recombinant Bone Morphogenetic Protein 8B (BMP8B)

Organism Species: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Gly20~His399

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P55105

Host: E. coli

Subcellular Location: Secreted.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 7.4

Predicted Molecular Mass: 46.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

G GH L S H P P H V F PQ R R L G V R E P RD MQ R E I R E V LG LP G R P R S R AP V G A A Q Q PA

SAPLFMLDLY RAMTDDSGGG TPQPHLDRAD LIMSFVNIVE RDRTLGYQEP HWKEFHFDLT

QIPAGEAVTA AEFRIYKEPS THPLNTTLHI SMFEVVQEHS NRESDLFFLD LQTLRSGDEG

WLVLDITAAS DRWLLNHHKD LGLRLYVETE DGHSIDPGLA GLLGRQAPRS RQPFMVGFFR

ANQSPVRAPR TARPLKKKQL NQINQLPHSN KHLGILDDGH GSHGREVCRR HELYVSFRDL

GWLDSVIAPQ GYSAYYCAGE CIYPLNSCMN STNHATMQAL VHLMKPDIIP KVCCVPTELS

AISLLYYDRN NNVILRRERN MVVQACGCH

[ REFERENCES ]

1.Zhao G.Q., Hogan B.L. (1996) Mech. Dev. 57:159-168.

2.Zhao G.Q., et al. (1996) Genes Dev. 10:1657-1669.

3.Ying Y., et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98:7858-7862.

4.Whittle A.J., et al. (2012) Cell 149:871-885.

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