15% SDS-PAGE

YB91355Ra01

Arachidonate-5-Lipoxygenase (ALOX5)

Organism: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

4th Edition (Revised in August, 2012)

[ DESCRIPTION ]

Rat ALOX5  kDa Protein Names: Arachidonate-5-Lipoxygenase

Synonyms: ALOX5

94 Species: Rat

66.2

Size: 100µg

45Source: Escherichia coli -derived

Subcellular Location: Cytoplasm; Nucleus matrix; Nucleus membrane; Peripheral

33 membrane protein.

26 [ PROPERTIES ]

Residues: Leu373~Leu535 (Accession # P12527), with N-terminal His-Tag.

20Grade & Purity: >95%, 20 kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in PBS, pH 7.4, containing 0.01% Sarcosyl, 5% sucrose.

14.Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

4Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 20.1 kDa

Predicted isoelectric point: 5.8

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months .

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-tag, its sequence is listed below.

MGHHHHHHSG SEF-LVSEVFG IAMYRQLPAV HPLFKLLVAH VRFTIAINTK AREQLNCEYG LFDKANATGG GGHVQMVQRA

VQDLTYSSLC  FPEAIKARGM  DNTEDIPYYF  YRDDGLLVWE AIQSFTTEVV  SIYYEDDQVV  EEDQELQDFV  KDVYVYGMRG

RKASGFPKSI KSREKL

[ REFERENCES ]

1.Balcarek J.M., et al. (1988) J. Biol. Chem. 263:13937-13941.

2.Minor W., et al. (1993) Biochemistry 32:6320-6323.

3.Drazen J.M., et al. (1999) Nat. Genet. 22:168-170.

4.Coreno A., et al. (2000) J. Allergy Clin. Immunol. 106:500-506.

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