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肌球蛋白重链14(MYH14)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation a
YBD454Hu01 100µg
Recombinant Myosin Heavy Chain 14, Non Muscle (MYH14)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Arg1069~Leu1331 (Accession # Q7Z406), with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Cell membrane;
Single-pass type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.4
Predicted Molecular Mass: 31.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-RK EEKGRQELEK LKRRLDGESS ELQEQMVEQQ QRAEELRAQL
GRKEEELQAA LARAEDEGGA RAQLLKSLRE AQAALAEAQE DLESERVART KAEKQRRDLG
EELEALRGEL EDTLDSTNAQ QELRSKREQE VTELKKTLEE ETRIHEAAVQ ELRQRHGQAL
GELAEQLEQA RRGKGAWEKT RLALEAEVSE LRAELSSLQT ARQEGEQRRR RLELQLQEVQ
GRAGDGERAR AEAAEKLQRA QAELENVSGA LNEAESKTIR L
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