YB90988Ra01

Cytochrome P450 2E1 (CYP2E1)

Organism: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

5th Edition (Revised in January, 2013)

[ DESCRIPTION ]

Protein Names: Cytochrome P450 2E1

Synonyms: CYP2E1, Cyp2e, Cyp2e-1

Species: Rat

Rat CYP2E1 kDa

Size: 100µg

Source: Escherichia coli-derived

Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane.

[ PROPERTIES ]

Residues: Arg126~Arg309 (Accession # P05182), with N-terminal His-Tag.

Grade & Purity: >95%, 25kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 23.1kDa

Predicted isoelectric point: 7.1

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-RRFSL SILRDWGMGK QGNEARIQRE AQFLVEELKK TKGQPFDPTF

LIGCAPCNVI  ADILFNKRFD YNDKKCLRLM SLFNENFYLL  STPWIQLYNN  FADYLRYLPG

SHRKIMKNVS EIKQYTLEKA KEHLQSLDIN CARDVTDCLL IEMEKEKHSQ EPMYTMENVS

VTLADLFFAG TETTSTTLR

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