YBH757Mu01 50ug

Recombinant Klotho (KL)

Organism Species: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Pro516~Phe955

Tags: N-terminal His-Tag

Accession: O35082

Host: E. coli

Subcellular Location: Cell membrane; Secreted.

Single-pass type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 8.3

Predicted Molecular Mass: 52.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

PLEGT FPCDFAWGVV DNYVQVDTTL SQFTDPNVYL WDVHHSKRLI KVDGVVAKKR

KPYCVDFSAI RPQITLLREM RVTHFRFSLD WALILPLGNQ TQVNHTVLHF YRCMISELVH

ANITPVVALW QPAAPHQGLP HALAKHGAWE NPHTALAFAD YANLCFKELG HWVNLWITMN

EPNTRNMTYR AGHHLLRAHA LAWHLYDDKF RAAQKGKISI ALQADWIEPA CPFSQNDKEV

AERVLEFDIG WLAEPIFGSG DYPRVMRDWL NQKNNFLLPY FTEDEKKLVR GSFDFLAVSH

YTTILVDWEK EDPMKYNDYL EVQEMTDITW LNSPSQVAVV PWGLRKVLNW LRFKYGDLPM

YVTANGIDDD PHAEQDSLRI YYIKNYVNEA LKAYVLDDIN LCGYFAYSLS DRSAPKSGFY

RYAANQFEPK PSMKHYRKII DSNGF

[ REFERENCES ]

1.Putaala H., et al. (2000) J. Am. Soc. Nephrol. 11:991-1001.

2.Kawachi H., et al. (2000) Kidney Int. 57:1949-1961.

3.Ahola H., et al. (1999) Am. J. Pathol. 155:907-913.

4.Luimula P., et al. (2000) Kidney Int. 58:1461-1468.

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