YB98293Hu01

Debranching Enzyme Homolog 1 (DBR1)

Organism: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

4th Edition (Revised in February, 2012)

[ DESCRIPTION ]

Human DBR1  kDa Protein Names: Debranching Enzyme Homolog 1

Synonyms: DBR1

Species: Human

Size: 5µg

Source: Escherichia coli-derived

Subcellular Location: Nucleus.

[ PROPERTIES ]

Residues: Gly243~Ala372 (Accession # Q9UK59), with N-terminal His-Tag.

20Grade & Purity: >97%, 16.2 kDa as determined by SDS-PAGE reducing conditions. Formulation: Supplied as lyophilized form in PBS, pH 7.4, containing 0.01% Sarcosyl, 5% sucrose.

14.Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

15% SDS-PAGE Predicted Molecular Mass: 16.2 kDa

Predicted isoelectric point: 4.8

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2- pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months .

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES]

The target protein is fused with N-terminal His-tag, its sequence is listed below.

MGHHHHHHSGSEF-GQTARATK  FLALDKCLPH RDFLQILEIE HDPSAPDYLE  YDIEWLTILR  ATDDLINVTG

RLWNMPENNG LHARWDYSAT EEGMKEVLEK LNHDLKVPCN FSVTAACYDP SKPQTQMQLI HRINPQTTEF

CA

[ REFERENCES ]

1.Kim JW., et al. (2000) Nucleic Acids Res 28(18): 3666–73.

2.Chapman KB., et al. (1991) Cell 65 (3): 483–92.

3.Arenas J., et al. (1987) J. Biol. Chem. 262(9): 4274–9.

4.Martin A., et al. (2002) J. Biol. Chem. 277(20): 17743–50.

5.Strausberg RL., et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903.

 您可以点击 下载产品说明书, 或直接联系我们订购.