The possible reasons that the actual band size differs from the predicted are as follows:

YBF642Ra01 100µg

Recombinant Phosphodiesterase 4B, cAMP Specific (PDE4B)

Organism Species: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Lys500~Thr736

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P14646

Host: E. coli

Subcellular Location: Cell periphery, cytoplasm.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 4.5

Predicted Molecular Mass: 31.1kDa

Accurate Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2.Relative charge: The composition of amino acids may affects the charge of the protein.

3.Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4.Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.

5.Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

K KQRQTLRKMV IDMVLATDMS KHMSLLADLK TMVETKKVTS SGVLLLDNYT DRIQVLRNMV

HCADLSNPTK SLELYRQWTD RIMEEFFQQG DKERERGMEI SPMCDKHTAS VEKSQVGFID

YIVHPLWETW ADLVQPDAQD ILDTLEDNRN WYQSMIPQSP SPPLDERSRD CQGLMEKFQF

ELTLEEEDSE GPEKEGEGPN YFSSTKTLCV IDPENRDSLE ETDIDIATED KSLIDT

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