YBB944Mu01 10µg

Recombinant Annexin A2 (ANXA2)

Organism Species: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PROPERTIES ]

Residues: Val46~Glu181 ((Accession # P07356), with two N-terminal Tags, His-tag and T7-tag.

Host: E. coli

Subcellular Location: Secreted. extracellular space, extracellular matrix basement membrane. Melanosome.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 9.0

Predicted Molecular Mass: 19.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.

MG S S HH H H H H SS G LV P R G S H MA SM TG G Q Q M GRG SE F - VK T K G VD E V TI V N IL

TNRSNVQRQD IAFAYQRRTK KELPSALKSA LSGHLETVIL GLLKTPAQYD ASELKASMKG

LGTDEDSLIE IICSRTNQEL QEINRVYKEM YKTDLEKDII SDTSGDFRKL MVALAKGRRA

E

[ REFERENCES ]

1.Saris C.J.M., et al. (1986) Cell 46:201-212.

2.Amiguet P., et al. (1990) Biochemistry 29:1226-1232.

3.Fey M.F., et al. (1996) Biochim. Biophys. Acta 1306:160-170.

4.Lennon N.J., et al. (2003) J. Biol. Chem. 278:50466-50473.

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