YBA011Rb01 50µg

Recombinant Brain Derived Neurotrophic Factor (BDNF)

Organism Species: Oryctolagus cuniculus (Rabbit)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PROPERTIES ]

Residues: Met16~Gly183

Tags: N-terminal His-Tag

Accession: G1SRK9

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 6.0

Predicted Molecular Mass: 20.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MVISY FGCMKAAPMK  EANVRGQGSL AYPGMRTHGT LESVNGPKTG  SRGLTSLADT

FEHVIEELLD EDQKVRPNEE NNKDADLYTS RVMLSSQVPL EPPLLFLLEE YKNYLDAANM

SMRVRRHSDP ARRGELSVCD SISEWVTAAD KKTAVDMSGG TVTVLEKVPV SKG

[ REFERENCES ]

1.Lindblad-Toh K., et al. (2011) Nature 478:476-482.

2.Robinson RC., et al. (1995) Biochemistry 34 (13): 4139–46.

3.Huang EJ., Reichardt LF. (2001) Annual Review of Neuroscience 24: 677–736.

4.Yamada K., Nabeshima T. (2003) Journal of Pharmacological Sciences 91 (4): 267–70.

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