YB90638Mu01

Visfatin (VF)

Organism: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

4th Edition (Revised in August, 2012)

[ DESCRIPTION ]

Protein Names: Visfatin

Synonyms: VF, Pbef1

Species: Mouse

Size: 100µg

Source: Escherichia coli -derived

Subcellular Location: Cytoplasm.

[ PROPERTIES ]

Residues: Met1~His491 (Accession # Q99KQ4), with N-terminal His-Tag.

Grade & Purity: >95%, 57 kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 57.0 kDa

Predicted isoelectric point: 6.7

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKVR KVKYEETVFY GLQYILNKYL

KGKVVTKEKI  QEAKEVYREH  FQDDVFNERG  WNYILEKYDG  HLPIEVKAVP  EGSVIPRGNV  LFTVENTDPE  CYWLTNWIET

ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGIALIKKY

YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV SRSTEAPLII

RPDSGNPLDT VLKVLDILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KKWSIENVSF GSGGALLQKL

TRDLLNCSFK  CSYVVTNGLG  VNVFKDPVAD PNKRSKKGRL SLHRTPAGNF  VTLEEGKGDL EEYGHDLLHT  VFKNGKVTKS

YSFDEVRKNA QLNIEQDVAP H

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