YB90780Ra02

Lactoferrin (LTF)

Organism: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

5th Edition (Revised in January, 2013)

[ DESCRIPTION ]

Rat LTF

Protein Names: Lactoferrin

Synonyms: LTF

Species: Rat

Size: 100µg

Source: Escherichia coli-derived

[ PROPERTIES ]

Residues: Cys64~Gly193 (Accession # D3ZAB1), with N-terminal His-Tag.

Grade & Purity: >95%, 18kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl.

Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 16.0kDa

Predicted isoelectric point: 8.6

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF- CIQEIMM RRADAMTLSG SAIFDFYFPY KLQPIAAEVY GTKEKPRIHY

YAVAVVKNSS DIRLNQLQGL KSCHAGFDTS AGWIAPLGAL RPYLNWDEKS VSLEEAVSKF

FSQSCVPGIS KSRFPRLCSL CAG

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