YBA780Po01 50µg

Recombinant Lactoferrin (LTF)

Organism Species: Sus scrofa; Porcine (Pig)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PROPERTIES ]

Residues: Val25~Glu348 (Accession # P14632), with two N-terminal Tags, His-tag and GST-tag.

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 8.7

Predicted Molecular Mass: 68.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID

GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV

DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK

KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR

GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-VRWCVI STAEYSKCRQ

WQSKIRRTNP MFCIRRASPT DCIRAIAAKR ADAVTLDGGL VFEADQYKLR PVAAEIYGTE

ENPQTYYYAV AVVKKGFNFQ LNQLQGRKSC HTGLGRSAGW NIPIGLLRRF LDWAGPPEPL

QKAVAKFFSQ SCVPCADGNA YPNLCQLCIG KGKDKCACSS QEPYFGYSGA FNCLHKGIGD

VAFVKESTVF ENLPQKADRD KYELLCPDNT RKPVEAFREC HLARVPSHAV VARSVNGKEN

SIWELLYQSQ KKFGKSNPQE FQLFGSPGQQ KDLLFRDATI GFLKIPSKID SKLYLGLPYL

TAIQGLRE

[ REFERENCES ]

1.Alexander L.J., et al. (1992) Anim. Genet. 23:251-256.

2.Lyndon J.P., et al. (1992) Biochim. Biophys. Acta 1132:97-99.

3.Hutchens T.W., et al. (1989) Biochim. Biophys. Acta 999:323-329.

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