5th Edition (Revised in January, 2013)

Rat LPO

YB90296Ra01

Lactoperoxidase (LPO)

Organism: Rattus norvegicus (Rat)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

[ DESCRIPTION ]

Protein Names: Lactoperoxidase

Synonyms: LPO

Species: Rat

Size: 100)g

Source: Escherichia coli-derived

[ PROPERTIES ]

Residues: Pro351~Glu589 (Accession # D4A400), with N-terminal His-Tag.

Grade & Purity: >95%, 31kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl.

Endotoxin Level: <1.0 EU per 1µg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 28.9kDa

Predicted isoelectric point: 6.3

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF- PCEVINATAG VPCFLAGDSR ASEQILLATS HTLFIREHNR

LARELSTLNP HWDGETLYQE TRKIMGAFIQ IITFRDYLPI LLGDEMQKWI PPYQGYNESV

DPRISNVFTF AFRFGHLEIP STVSRLDENY QPWGSEPELP LHTVFFNTWR LVKDGGIDPL

VRGLLAKKAK LMHQDRMMTG ELRNKLFQPT HTIHGFDLAS INIQRCRDHG MPGYNSWRAF

CGLSQPKTLE ELSAVMENE

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