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LIG4 Polyclonal Antibody
LIG4
货号：YBAP11973 |
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规格： 50ul/100ul/200ul |
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价格： 980/1280/1890 |
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| Immunogen： | Recombinant protein of human LIG4 | ||
Synonym: |
LIG4 | ||
| Calculated MW: | 104kDa | ||
| Observed MW: | Refer to figures | ||
Source: |
Rabbit | ||
Isotype: |
IgG | Reactivity: H,M,R; Clonality: Polyclonal  |
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Application: |
WB | ||
Purify: |
Affinity purification | ||
Concentration: |
3.78mg/ml | ||
Storage Buffer: |
PBS with 0.05% sodium azide, 50% glycerol, pH7.3 | ||
Storage: |
Store at -20℃. Avoid freeze / thaw cycles. |
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| Western blot analysis of K562, Jurkat and MCF7 cell, using LIG4 Polyclonal Antibody at dilution of 1:500 |
Protocols： |
Buffer-Formulation WB-Guide IHC-Guide IP-Guide IF-Guide |
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Background: |
Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.
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