Alternative Names:

CBX5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha;

Application:

WB IHC ICC/IF

Reactivity:

Human,Mouse

Dilution:

WB 1:1000 IHC 1:200 ICC/IF 1:300

	Western blot detection of HP1 alpha in MCF7,293T,HelaNE,3T3,C6,COS7 and Mouse brain lysates and using HP1 alpha mouse mAb (1:1000 diluted).Predicted band size: 22KDa.Observed band size: 22KDa
	Immunohistochemical analysis of paraffin-embedded human breast carcinoma with HP1 alpha mouse mAb (3G2-H10-A6, 1:400 diluted ),showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrat buffer(pH6.0).
	Immunofluorescent analysis stain of Hela using HP1 alpha mouse mAb (1:300).

Purification:

Affinity-chromatography

Specificity:

HP1-α Mouse mAb detects endogenous levels of total HP1-α

Immunogen:

Purified recombinant human HP1 alpha protein fragments expressed in E.coli.

Description:

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR).

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.