Alternative Names:

NPM isoform 1; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; nucleophosmin;

Application:

WB

Reactivity:

Human,Mouse

Dilution:

WB 1:1000

	Western blot detection of NPM1 in Hela,Jurkat and 3T3 cell lysates using NPM1 mouse mAb (1:1000 diluted).Predicted band size:33KDa.Observed band size:38KDa.

Purification:

Affinity-chromatography

Specificity:

NPM1 Mouse mAb detects endogenous levels of total NPM1

Immunogen:

Purified recombinant full length of human NPM1 protein expressed in E.coli.

Description:

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.