Alternative Names:

Octamer binding protein 3; Octamer binding protein 4; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4;

Application:

WB

Reactivity:

Mouse

Dilution:

WB 1:1000

	Western blot detection of POU5F1/OCT4 in F9 cell lysates using POU5F1/OCT4 mouse mAb (1:1000 diluted).Predicted band size: 45KDa.Observed band size: 45KDa.

Purification:

Affinity-chromatography

Specificity:

POU5F1/OCT4 Mouse mAb detects endogenous levels of total POU5F1/OCT4

Immunogen:

Purified recombinant mouse POU5F1/OCT4 protein fragments expressed in E.coli.

Description:

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3').Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1,FGF4,UTF1 and ZFP206.Critical for early embryogenesis and for embryonic stem cell pluripotency.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.