Alternative Names:

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1; COX4I1; COX4;

Application:

WB IHC ICC/IF IP

Reactivity:

Human,Mouse,Rat,Hamster,Goat,Monkey

Dilution:

WB 1:1000 IHC 1:50 ICC/IF 1:150 IP FC 1:100

	Western blot detection of COX IV in Goat muscle,CHO-k1,COS7,3T3,Hela,C6 and K562 cell lysates using COX IV mouse mAb (1:5000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
	Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with COX IV Mouse mAb (4D11-B3-E8,1:50 diluted),showing cytoplasm localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).
	Immunofluorescent analysis of HeLa cells using anti-COX IV mouse mAb diluted 1:150.
	Immunoprecipitation analysis of Hela cell lysates using COX IV mouse mAb.
	Flow Cytometry analysis of K562 cells stained with COX4 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.

Purification:

Affinity-chromatography

Specificity:

COX IV Mouse mAb detects endogenous levels of total COX IV

Immunogen:

A synthetic peptide corresponding to carboxyl terminal residues of human COX IV

Description:

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.