Alternative Names:

bA528A10.3; E3 ubiquitin-protein ligase RNF144B; IBR domain containing 2; IBR domain-containing protein 2; IBRDC2; KIAA0161; MGC71786; p53-inducible RING finger protein; P53RFP; R144B; ring finger 144B; RING finger protein 144B; RNF144B

Application:

WB

Reactivity:

Human, Mouse

Dilution:

WB 1:500~1:1000

	Western blot analysis of extracts from HeLa cells, using RNF144B antibody.

Purification:

Affinity-chromatography

Specificity:

RNF144B Antibody detects endogenous levels of total RNF144B

Immunogen:

A synthesized peptide derived from human RNF144B

Description:

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.