Alternative Names:

86 kDa subunit of Ku antigen, ATP-dependent DNA helicase II, 80 kDa subunit, CTC box binding factor 85 kDa subunit, CTC85, CTCBF, DNA-repair protein XRCC5, G22P2, KU86, Lupus Ku autoantigen protein p86, Nuclear factor IV, TLAA, Thyroid- lupus autoantigen,

Application:

WB,IHC,IF

Reactivity:

Human,Mouse,Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF 1:100-1:500

	Western blot analysis of Ku70/80 expression in LoVo cell lysate

Purification:

Affinity-chromatography

Specificity:

Ku70/80 Antibody detects endogenous levels of total Ku70/80

Immunogen:

A synthesized peptide derived from human Ku70/80

Description:

The Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. The Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.