Alternative Names:

CUL-1; CUL1; cullin 1; Cullin-1; MGC149834; MGC149835

Application:

WB IHC IF/ICC

Reactivity:

Human, Mouse

Dilution:

WB 1:500~1:3000 IHC 1:50~1:200 IF/ICC 1:100~1:500

	Western blot analysis on HeLa cell lysate using Cullin 1 Antibody

Purification:

Affinity-chromatography

Specificity:

Cullin 1 antibody detects endogenous levels of total Cullin 1

Immunogen:

A synthesized peptide derived from human Cullin 1

Description:

CUL1 Core component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.