Alternative Names:

CUL-2; CUL2; cullin 2; Cullin-2; MGC131970

Application:

WB IHC IF/ICC

Reactivity:

Human, Mouse

Dilution:

WB 1:500~1:3000 IHC 1:50~1:200 IF/ICC 1:100~1:500

	Western blot analysis on LOVO cell lysate using Cullin 2 Antibody

Purification:

Affinity-chromatography

Specificity:

Cullin 2 antibody detects endogenous levels of total Cullin 2

Immunogen:

A synthesized peptide derived from human Cullin 2

Description:

CUL2 Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.