Alternative Names:

ALPS2; Apoptotic protease Mch-4; CASP-10; CASP10; CASPA; caspase 10, apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine protease; Caspase-10; Caspase-10 subunit p12; Caspase-10 subunit p23/17; FADD-like ICE2; FAS-associated death

Application:

WB IHC IF/ICC

Reactivity:

Human

Dilution:

WB 1:500~1:3000 IHC 1:50~1:200 IF/ICC 1:100~1:500

	Western blot analysis on HeLa cell lysate using Caspase 10 Antibody

Purification:

Affinity-chromatography

Specificity:

Caspase 10 antibody detects endogenous levels of total Caspase 10

Immunogen:

A synthesized peptide derived from human Caspase 10

Description:

Casp10 Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Belongs to the peptidase C14A family. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit. Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. 6 isoforms of the human protein are produced by alternative splicing.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.