Alternative Names:

CCNE; CCNE1; cyclin E1; cyclin Es; cyclin Et; G1/S-specific cyclin-E1

Application:

WB IHC IF/ICC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500~1:3000 IHC 1:50~1:200 IF/ICC 1:100~1:500

	Western blot analysis on HeLa cell lysate using Cyclin E1 Antibody

Purification:

Affinity-chromatography

Specificity:

Cyclin E1 antibody detects endogenous levels of total Cyclin E1

Immunogen:

A synthesized peptide derived from human Cyclin E1

Description:

CCNE1 a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.