Alternative Names:

TPX2, C20orf2, DIL-2, DIL2, HCA519, GD:C20orf1, p100, Protein fls353, Targeting protein for Xklp2, C20orf1, FLS353, HCTP4, REPP86

Application:

WB IHC

Reactivity:

Human Mouse

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200

	Western blot analysis TPX2 using RAW264.7 whole cell lysates.

Purification:

Affinity-chromatography

Specificity:

TPX2 Antibody detects endogenous levels of total TPX2

Immunogen:

A synthesized peptide derived from human TPX2

Description:

Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.