Alternative Names:

cNOS; Constitutive NOS; EC-NOS; ECNOS; Endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase, endothelial; NOS type III; NOS3; NOSIII

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB: 1:500~1:2000 IHC 1:50~1:200

	Western blot analysis on K562 cell lysate using eNOS Antibody

Purification:

Affinity-chromatography

Specificity:

eNOS antibody detects endogenous levels of total eNOS

Immunogen:

A synthesized peptide derived from human eNOS

Description:

eNOS endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.