Alternative Names:

APFL; APLF; Aprataxin and PNK-like factor; aprataxin and PNKP like factor; aprataxin- and PNK-like factor; Apurinic-apyrimidinic endonuclease APLF; C2orf13; FLJ16593; MGC47799; PALF; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA p

Application:

WB IHC

Reactivity:

Human, Mouse

Dilution:

WB 1:500~1:3000 IHC 1:50~1:200

	Western blot analysis on rat heart cell lysate using APLF Antibody

Purification:

Affinity-chromatography

Specificity:

APLF antibody detects endogenous levels of total APLF

Immunogen:

A synthesized peptide derived from human APLF

Description:

APLF Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic- apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Belongs to the APLF family. Interacts with LIG4, PARP1, XRCC1, XRCC4 and XRCC5.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.