Alternative Names:

E2F transcription factor 4, p107/p130-binding; E2F-4; E2F4; p107/p130-binding protein; Transcription factor E2F4

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500~1:3000 IHC 1:50~1:200

	Western blot analysis on COS7 cell lysate using E2F4 Antibody

Purification:

Affinity-chromatography

Specificity:

E2F4 antibody detects endogenous levels of total E2F4

Immunogen:

A synthesized peptide derived from human E2F4

Description:

E2F4 Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein. Belongs to the E2F/DP family. Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.