Alternative Names:

80 kDa MCM3-associated protein; FLJ44336; FLJ45306; GANP; germinal center-associated nuclear protein; KIAA0572; MAP80; MCM3 import protein; MCM3 minichromosome maintenance deficient 3 associated protein; MCM3A; MCM3AP; minichromosome maintenance complex c

Application:

WB IHC

Reactivity:

Human, Mouse

Dilution:

WB 1:500~1:3000 IHC 1:50~1:200

	Western blot analysis on NIH-3T3 cell lysate using GANP Antibody

Purification:

Affinity-chromatography

Specificity:

GANP antibody detects endogenous levels of total GANP

Immunogen:

A synthesized peptide derived from human GANP

Description:

GANP is a member of the SAC3 family of nuclear export factors. In combination with ENY2, PCID2 and centrins, forms the TREX-2 complex that links transcription/ processing with nuclear mRNA export. GANP, required to recruit ENY2 to nuclear pore complexes (NPCs), requires both its CID and MCM3AP domains, together with nucleoporin Nup153, for recruitment to NPCs. The MCM3AP domain, corresponding to the carboxy-terminal 721 amino acids of GANP, shows homology with the Gcn5-related N acetyltransferase superfamily. The protein MCM3AP is encoded entirely within the 3¿ region of the GANP gene. MCM3AP transcription begins within intron 16 of GANP immediately upstream of the first MCM3AP exon, where NF-IL6, AP-1, NF-¿B, and p53 consensus transcription factor binding sites are located.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.