Alternative Names:

AR-JP; E3 ubiquitin ligase; E3 ubiquitin-protein ligase parkin; LPRS2; PARK2; parkin; parkin 2; Parkinson disease (autosomal recessive, juvenile) 2, parkin; Parkinson disease protein 2; Parkinson juvenile disease protein 2; PDJ; PRKN; PRKN2

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500~1:3000 IHC 1:50~1:200

	Western blot analysis on HeLa cell lysate using Parkin Antibody

Purification:

Affinity-chromatography

Specificity:

Parkin antibody detects endogenous levels of total Parkin

Immunogen:

A synthesized peptide derived from human Parkin

Description:

PARK2 Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP, SEPT5 and AIMP2. May play a more general role in the ubiquitin proteasomal pathway by participating in the removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of PARK2.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.