Alternative Names:

DD5; E3 identified by differential display; E3 ubiquitin protein ligase, HECT domain containing, 1; E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase, HECT domain-containing 1; EDD; EDD1; FLJ11310; hHYD; HYD; Hyperplastic discs protein homolog

Application:

WB IHC

Reactivity:

Human, Mouse

Dilution:

WB 1:500~1:3000 IHC 1:50~1:200

	Western blot analysis on A549 cell lysate using EDD Antibody

Purification:

Affinity-chromatography

Specificity:

EDD antibody detects endogenous levels of total EDD

Immunogen:

A synthesized peptide derived from human EDD

Description:

UBR5 E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.