Alternative Names:

HEC; HEC1; Highly expressed in cancer protein; highly expressed in cancer, rich in leucine heptad repeats; HsHec1; hsNDC80; kinetochore associated 2; Kinetochore protein Hec1; Kinetochore protein NDC80 homolog; Kinetochore-associated protein 2; KNTC2; NDC

Application:

WB IF/ICC

Reactivity:

Human, Mouse

Dilution:

WB 1:500~1:3000 IF/ICC 1:100~1:500

	Western blot analysis on Jurkat,A549 and HuvEc cell lysate using HEC1 Antibody

Purification:

Affinity-chromatography

Specificity:

HEC1 antibody detects endogenous levels of total HEC1

Immunogen:

A synthesized peptide derived from human HEC1

Description:

HEC1 plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.