Alternative Names:

BA2R, CCG1, Cell cycle gene 1 protein, EC 2.7.11.1, P250, T2D1, TAF2A, TAFII-250, TAFII250, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, kinase TAF1

Application:

IHC

Reactivity:

Human, Mouse,

Dilution:

IHC 1:50-1:200

Purification:

Affinity-chromatography

Specificity:

TAF1 Antibody detects endogenous levels of TAF1

Immunogen:

A synthesized peptide derived from human TAF1

Description:

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Two transcripts encoding different isoforms have been identified for this gene.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.