Alternative Names:

CDK2/cyclin A-associated protein p45; cyclin A/CDK2-associated protein p45; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; FBL1; FBXL1; FLB1; MGC1366; p45skp2; S-phase kinase-associated protein 2; S-phase kinase-ass

Application:

WB IHC IF/ICC

Reactivity:

Human,

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

	Western blot analysis on 293 cell lysate using SKP2/p45 Antibody

Purification:

Affinity-chromatography

Specificity:

SKP2/p45 Antibody detects endogenous levels of SKP2/p45

Immunogen:

A synthesized peptide derived from human SKP2/p45

Description:

SKP2 an F-box protein of the SCF (SKP1-CUL1-F- box protein) E3 ubiquitin ligase system. The F-box component of the SCF complex is responsible for recognizing different substrates for ubiquitination. The SCF E3 complex mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Three alternatively-spliced isoforms have been described.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.