Alternative Names:

NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65

Application:

WB IHC IF/ICC

Reactivity:

Human, Mouse,

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

	Western blot analysis on HeLa cell lysate using NF-κB p65 Antibody

Purification:

Affinity-chromatography

Specificity:

NF-κB p65 Antibody detects endogenous levels of NF-κB p65

Immunogen:

A synthesized peptide derived from human NF-κB p65

Description:

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.