Alternative Names:

H3/a; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31; HIST1H3A; histone H3.1

Application:

WB IF

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500~1:1000 IF 1:200

	Western blot analysis of extracts from Raw264.7 cells, treated with TSA 400nM 24h, using Acetyl-Histone H3 (Lys23) Antibody. The lane on the left is treated with the synthesized peptide.

Purification:

Affinity-chromatography

Specificity:

Acetyl-Histone H3 (Lys23) Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine23.

Immunogen:

The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys23.

Description:

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.