Alternative Names:

H4F2; HIST; HIST1H4A; Histone H4

Application:

WB IHC IF

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 IF 1:200

	Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Acetyl-Histone H4 (Lys12) Antibody. The lane on the left is treated with the synthesized peptide

Purification:

Affinity-chromatography

Specificity:

Acetyl-Histone H4 (Lys12) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at lysine12.

Immunogen:

The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys12.

Description:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.