Alternative Names:

H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody

Application:

WB IHC IF

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 IF 1:200

	Western blot analysis of extracts from HeLa cells, treated with TSA 400nM 24h, using Acetyl-Histone H3 (Lys18) Antibody. The lane on the left is treated with the synthesized peptide.

Purification:

Affinity-chromatography

Specificity:

Acetyl-Histone H3 (Lys18) Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine18.

Immunogen:

The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys18.

Description:

H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.