Alternative Names:

Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53

Application:

WB IF

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500~1:1000 IF 1:200

	Western blot analysis of extracts from HeLa cells, treated with TSA 400nM 24h, using Acetyl-p53 (Lys317) Antibody. The lane on the left is treated with the synthesized peptide.

Purification:

Affinity-chromatography

Specificity:

Acetyl-p53 (Lys317) Antibody detects endogenous levels of total p53 protein only when acetylated at lysine317.

Immunogen:

The antiserum was produced against synthesized peptide derived from human p53 around the acetylated site of Lys317.

Description:

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.