Alternative Names:

H4F2, HIST, HIST1H4A, Histone H4.AC-H4K5,H4K5

Application:

WB IHC IF/ICC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

	Western blot analysis of Acetyl-Histone H4 phosphorylation expression in TSA treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Acetyl-Histone H4 (Lys5) Antibody detects endogenous levels of Acetyl-Histone H4 only when phosphorylated at Lys5

Immunogen:

A synthesized peptide derived from human Acetyl-Histone H4 around the phosphorylation site of Lys5

Description:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.