Alternative Names:

H3 histone, family 3A; H3 histone, family 3B (H3.3B); H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3,AC-H3K9,h3k9

Application:

WB IHC IF/ICC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

	Western blot analysis of Acetyl-Histone H3 phosphorylation expression in TSA treated RAW264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Acetyl-Histone H3(Lys9) Antibody detects endogenous levels of Acetyl-Histone H3 only when phosphorylated at Lys9

Immunogen:

A synthesized peptide derived from human Acetyl-Histone H3 around the phosphorylation site of Lys9

Description:

H3F3A Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.