Alternative Names:

E2F-2

Application:

WB

Reactivity:

Human, Mouse

Dilution:

WB 1:500-1:1000

	Western blot analysis of extracts from Hela and K562 cells using E2F-2 Antibody

Purification:

Affinity-chromatography

Specificity:

The antibody detects endogenous level of total E2F-2 protein.

Immunogen:

Peptide sequence around aa.425~429(L-F-D-S-Y) derived from Human E2F-2.

Description:

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.