Alternative Names:

BRG1; SNF2; SWI2; RTPS2; BAF190

Application:

WB

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:1000

	Western blot analysis of extracts from 293 and HepG2 cells using Brg1 Antibody

Purification:

Affinity-chromatography

Specificity:

The antibody detects endogenous level of total Brg1 protein.

Immunogen:

Peptide sequence around aa.701~705(V-D-A-R-H) derived from Human Brg1.

Description:

Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.