Alternative Names:

I-kappa-B-alpha, IKBA, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappaB inhibitor alpha, NFKBI, NFKBIA, RL/IF-1

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of IkappaB-alpha expression in TNF-α treated MCF7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

IkappaB-alpha Antibody detects endogenous levels of total IkappaB-alpha

Immunogen:

A synthesized peptide derived from human IkappaB-alpha

Description:

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle