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Details
Alternative Names:
NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65
Application:
WB IHC
Reactivity:
Human, Mouse, Rat
Dilution:
WB 1:500-1:1000 IHC 1:50-1:200 IF 1:200
Western blot analysis of NF-kappaB p65 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. | |
Purification:
Affinity-chromatography
Specificity:
NF-kappaB p65 Antibody detects endogenous levels of total NF-kappaB p65
Immunogen:
A synthesized peptide derived from human NF-kappaB p65
Description:
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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