Alternative Names:

CASP9_HUMAN antibody Caspase 9 apoptosis related cysteine peptidase antibody Caspase 9 Dominant Negative antibody Caspase 9c antibody Caspase-9 antibody Caspase-9 subunit p10 antibody

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of Caspase 9 (Cleaved-Asp353) expression in NIH/3T3 cells treated with etoposide

Purification:

Affinity-chromatography

Specificity:

Caspase 9 (Cleaved-Asp353) Antibody detects endogenous levels of total Caspase 9 (Cleaved-Asp353)

Immunogen:

A synthesized peptide derived from human Caspase 9 (Cleaved-Asp353)

Description:

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.