Alternative Names:

Motch B, NOTC2, NOTCH2, NTC2, Neurogenic locus notch homolog protein 2 precursor, hN2

Application:

WB,IHC,IF

Reactivity:

Human,Mouse,Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF 1:100-1:500

	Western blot analysis of Notch 2 (Cleaved-Asp1733) expression in293 cell extract treated with TNF-alpha

Purification:

Affinity-chromatography

Specificity:

Notch 2 (Cleaved-Asp1733) Antibody detects endogenous levels of total Notch 2 (Cleaved-Asp1733)

Immunogen:

A synthesized peptide derived from human Notch 2 (Cleaved-Asp1733)

Description:

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC)

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.