Alternative Names:

MAPK3; MK03; MNK1; p44-ERK1; P44-ER; ERK-1; ERK1; ERT2; kinase ERK1; MAP kinase 1; MAPK 1;

Application:

WB IHC ICC/IF FC

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:100 FC 1:40

	Western blot analysis of Phospho- Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in A431 cell lysate treated with EGF.

Purification:

Affinity-chromatography

Specificity:

Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Antibody detects endogenous levels of total Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187)

Immunogen:

A synthesized peptide derived from human Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187)

Description:

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.