Alternative Names:

CASP3; Caspase-3; APOPAIN; CPP32; CPP32B; SCA-1;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Mouse

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of pro Caspase 3 in HeLa cell lysate.
	Immunofluorescent analysis of A673 cells, using pro Caspase 3 Antibody.

Purification:

Affinity-chromatography

Specificity:

pro Caspase 3 Antibody detects endogenous levels of total pro Caspase 3

Immunogen:

A synthesized peptide derived from human pro Caspase 3

Description:

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.